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KMID : 0384119860060020457
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Korean Journal of Clinical Pathology
1986 Volume.6 No. 2 p.457 ~ p.461
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A Simultaneous Assay for T-Cells and B-Cells Using Immunobead Method
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趙顯燦/Cho, Hyoun Chan
趙英淑/李揆晩/朴贊正/朴英毅/Cho, Young Sook/Lee, Kyu Man/Park, Chan Jeong/Park, Young Eu
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Abstract
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Clinically, the enumeration of lymphocyte subpopulations has become an aid of L e diagnosis of immunological diseases and of the evaluation of immunocompetence in a variety of disease states. Traditionally immunofluorescent technique commonly has been used to identify B-cells, while spontaneous rosetting with sheep erythrocytes (E-rosette formation) is used to identify T-cells. ´In actual practice, however, this techniques have several disadvantages and are sensitive !to-procedural variation.
The authors report here the result of our analyses using a new simultaneons assay for T-and B-cells known as the Quantigen T and B Cell Assay (Bio-Rad Laboratories)
On lymphocyte samples isolated from the blood of 34 normal individuals, % T-cell is 79.10±3.34 (mean±standard deviation), % B-cell 9.55±3.06, and % null. cell 11. 64±3.34. The immunobead assay was also performed on abnormal specimens including burn and hepatitis patients. The data obtained reveal characteristically decreased percentage of T cells.
It is believed that the immunobead method has several distinct advantages. But occasionally the differentiation of yellow and clear beads is difficult and platelet or monocyte contamination becomes a problem. The authors has found, however, that by adding 0.3% acetoorcein to the original assay at the last step more sharp distinction is obtained. With this dye the yellow beads turn deep yellow, while the colorless remains unchanged and also monocytes which contaminate lymphocyte preparations are identified more easily.
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